두경부악성종양세포주의 항암제감수성 시험에 관한 실험적 연구
Chemosensitivity of cancer cells to antioancer drugs using dye exclusion assay, [3H] thymidine incorporation, and clonogenic assay
진우정,
소속 상세정보
진우정 ( ) - 전북대학교
KMID : 0360119930150010035
Abstract
the in vitro predictive tests in cancer chamotherapy of cancer cell lines to anticancer drugs were determined using novel dye exclusion assay [NDEA], [3H] thymidine incorporation, and clonogenic assay [CA>. Antitumor effect of Bleomycin,
Cis-platin,
Vinblastine, Methotrexate to Hep-2, B16 cell lines using rapid assays was compared with [CA> in this study.
In dye exclusion assay of B 16 cell line, cancer cells were sensitive to Bleomycin at all concentrations, to Vinblastine at the level of peak plasma concentration [PPC], ×1/10 [PPC](P<0.05). and bleomycin revealed relatively good cytotoxicity
than
that
of CDDP and vinblastine at ×10 [PPC], (P<0.05).
Hep-2 cell swere resistive to methotrexate at the level of ×100 [PPC] (P<0.05)
In [3H] thymidine incorporation assay, B 16 cells were sensitive to Bleomycin, CDDP, Vinblastine at the level of [PPC], ×10 [PPC] (P<0.01).
Dose-dependent drugs of bleomycin, CDDP were more sensitive than Vinblastine at high concentration (P<0.05).
In clonogenic assay, Hep-2 cell line was sensitive to three drugs of all concentrations except ×1/10 [PPC] of CDDP. B 16 cell line was sensitive to all drugs (P<0.01).
In comparison of chemosensitivity tests among three assays, the results were correlated (γ=0.99, P<0.05).
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